| 论文作者 |
Gong, AD; Hong, R; Wang, GZ; Liu, JR; Li, SQ; Yang, P; Zhang, YM; Yang, G; Chen, Z; Wu, AB; He, WJ |
| 摘要 |
BACKGROUND Aspergillus flavus and the produced carcinogenic aflatoxins pose severe threats to food safety and human health. The control of A. flavus and aflatoxins have become a focal point for investigators across the world.RESULT Bacillus safensis TR-47 produced volatile compounds, significantly inhibit the mycelial growth and conidia germination of A. flavus, and completely prevent A. flavus infection and aflatoxins contamination in peanut kernels during storage. Moreover, TR-47 exhibited broad-spectrum antifungal activity against six other important phytopathogenic fungi. Gas chromatography-tandem mass spectrometry analysis revealed that the volatiles produced by TR-47 included dimethyl disulfide (DMDS), 2-heptanone (2-HP), 6-methyl-2-heptanone (MH), and dimethyl trisulfide (DMTS). These compounds exhibited great inhibitory effect on A. flavus with the minimum inhibitory concentration ranging from 10 to 200 mu L/L (compound volume/airspace volume). Transcriptomic analysis indicated that significantly differentially expressed genes were enriched in steroid biosynthesis, fatty acid degradation/metabolism, biosynthesis of unsaturated fatty acids, tyrosine/tryptophan metabolism and metabolic pathways, and so forth. Cellular staining further confirmed that MH triggered reactive oxygen species bursts, altered cell permeability, induced cell membrane damage, and ultimately led to cell death of A. flavus.CONCLUSION B. safensis TR-47 was capable of producing three antifungal volatiles including DMDS, MH and DMTS, which exhibited significant inhibitory effects on the growth, pathogenicity, and aflatoxins production of A. flavus. The inhibitory mechanism of MH was highly related to the damage of cell membrane of A. flavus. TR-47 and these volatiles were found to be promising biological materials for the production of biocontrol agents. (c) 2025 Society of Chemical Industry. |
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