| Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum | |
| 论文作者 | Lei, Y; Liu, YX; Wang, ML; Yuan, NN; Hou, YJ; Ding, LJ; Zhu, ZY; Wu, ZH; Li, C; Zheng, MY; Zhang, RY; Gomes, ARR; Xu, YF; Luo, ZK; Liu, Z; Chai, QW; Misery, P; Zhong, YQ; Song, XX; Lamy, C; Cui, W; Yu, Q; Fang, J; An, YJ; Tian, Y; Liu, YW; Sun, X; Wang, RQ; Li, HH; Song, JJ; Tan, X; Wang, H; Wang, SW; Han, L; Zhang, Y; Li, SY; Wang, KX; Wang, GL; Zhou, WQ; Liu, JF; Yu, C; Zhang, SZ; Chang, LT; Toplanaj, D; Chen, MN; Liu, JB; Zhao, Y; Ren, BY; Shi, HY; Zhang, H; Yan, HT; Ma, JY; Wang, LN; Li, Y; Zuo, YC; Lu, LJ; Gu, LQ; Li, ST; Wang, YQ; He, YY; Li, SK; Zhang, Q; Lu, YB; Dou, YN; Liu, Y; Zhao, AQ; Zhang, MY; Zhang, XY; Xia, Y; Zhang, W; Cao, HT; Lu, ZY; Yu, ZX; Li, X; Wang, XF; Liang, ZF; Xu, SJ; Liu, CR; Zheng, CH; Xu, C; Liu, ZY; Li, CY; Sun, YG; Xu, X; Dehay, C; Vezoli, J; Poo, MM; Yao, JH; Liu, LQ; Wei, W; Kennedy, H; Shen, ZM |
| 期刊/会议名称 | CELL |
| 论文年度 | 2025 |
| 论文类别 | |
| 摘要 | Claustrum orchestrates brain functions via its connections with numerous brain regions, but its molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, with most glutamatergic neurons similar to deep-layer insular neurons. Comparison of macaque, marmoset, and mouse transcriptomes revealed macaque-specific cell types. Retrograde tracer injections at 67 cortical and 7 subcortical regions defined four distinct distribution zones of retrogradely labeled claustral neurons. Joint analysis of whole-brain connectivity and single-cell spatial transcriptome showed that these four zones containing distinct compositions of glutamatergic (but not GABAergic) cell types preferentially connected to specific brain regions with a strong ipsilateral bias. Several macaque-specific glutamatergic cell types in ventral vs. dorsal claustral zones selectively co-projected to two functionally related areas-entorhinal cortex and hippocampus vs. motor cortex and putamen, respectively. These data provide the basis for elucidating the neuronal organization underlying diverse claustral functions. |
| 卷 | 188 |
| 影响因子 | 42.5 |
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