| Bilayer tissue-engineered skin loaded with confining forces enhances epidermal proliferation to promote full-thickness wound healing |
| 论文作者 |
Xu, C; Zhang, ZQ; Jin, QH; Cao, GB; Liu, ZH; Wan, JM; Ji, PX; Xu, L; Ju, JH; Le, YY; Hou, RX; Fu, Y; Zhang, GL |
| 期刊/会议名称 |
MATERIALS TODAY COMMUNICATIONS |
| 论文年度 |
2025 |
| 论文类别 |
|
| 摘要 |
Tissue-engineered skin is an ideal material for clinical use in wound repair. However, the epidermal layer of tissue-engineered skin has limited function owing to the inability to maintain its proliferative potential and weak antimicrobial properties, posing a challenge to its widespread clinical application in repairing skin defects. In this study, a bioink formulation containing 1 % gelatin methacryloyl (GelMA) and 0.5 % chitosan methacryloyl (CSMA) was used to develop a bilayer tissue-engineered skin material containing a dermal layer composed of human dermal fibroblasts and an epidermal layer composed of human keratinocytes. The bioprinted tissues showed antimicrobial properties with 85 % effectiveness in hydrogel antibacterial experiment, biocompatibility of over 89 % in cell viability test, and good mechanical properties. In vivo experiments further showed that the cell-inoculated GelMA-CSMA scaffolds cultured for 10 days maintained the proliferative potential of the epidermal layer of the bioprinted constructs under a confined force after transplantation to skin tissue defects in nude mice. Functional tests demonstrated that these scaffolds facilitate wound repair by promoting epidermal growth, collagen deposition, and skin appendage formation. Thus, bilayer tissue-engineered skin with mechanical stimulation loading offers an effective material for the treatment of skin defects, providing a new approach to clinical wound healing. |
| 卷 |
47 |
| 影响因子 |
4.5 |
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